FIGURE

Figure 5

ID

ZDB-FIG-230211-134

Publication

Morgan et al., 2023 - ahctf1 and kras mutations combine to amplify oncogenic stress and restrict liver overgrowth in a zebrafish model of hepatocellular carcinoma

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Figure 5

ahctf1 heterozygosity amplifies cell death in dox-treated TO(kras^G12V)^T/+ hepatocytes in the presence and absence of Tp53.

(a) Representative maximum intensity projection images of Annexin 5-mKate fluorescence (white puncta), indicating cells undergoing apoptosis in TO(kras^G12V)^T/+ livers of the indicated ahctf1 and tp53 genotypes. Scale bar 25 µm. (b) Quantification of the density of Annexin 5 fluorescent foci in TO(kras^G12V)^T/+ livers of the indicated ahctf1 and tp53 genotypes (n ≥ 11). (c) RT-quantitative PCR (RT-qPCR) analysis of the specified mRNAs in TO(kras^G12V)^T/+ micro-dissected livers of the indicated ahctf1 and tp53 genotypes (n = 3 biological replicates). Data are expressed as mean ± standard error of the mean (SEM). Significance was calculated using a one-way analysis of variation (ANOVA) with Tukey’s multiple comparisons test.

Expression Data

Genes:	badb bbc3 mdm2 mKate2 pmaip1
Fish:	gz32Tg/+ ahctf1^ti262c/+; gz32Tg/+ gz32Tg/+; uq24rpTg/uq24rpTg tp53^zdf1/zdf1; gz32Tg/+ ahctf1^ti262c/+; gz32Tg/+; uq24rpTg/uq24rpTg ahctf1^ti262c/+; tp53^zdf1/zdf1; gz32Tg/+ tp53^zdf1/zdf1; gz32Tg/+; uq24rpTg/uq24rpTg ahctf1^ti262c/+; tp53^zdf1/zdf1; gz32Tg/+; uq24rpTg/uq24rpTg
Condition:	chemical treatment by environment: doxycycline
Anatomical Terms:	hepatocyte apoptotic body liver
Stage:	Days 7-13

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	ahctf1^ti262c/+; gz32Tg/+ gz32Tg/+; uq24rpTg/uq24rpTg tp53^zdf1/zdf1; gz32Tg/+ ahctf1^ti262c/+; gz32Tg/+; uq24rpTg/uq24rpTg ahctf1^ti262c/+; tp53^zdf1/zdf1; gz32Tg/+ tp53^zdf1/zdf1; gz32Tg/+; uq24rpTg/uq24rpTg ahctf1^ti262c/+; tp53^zdf1/zdf1; gz32Tg/+; uq24rpTg/uq24rpTg
Condition:	chemical treatment by environment: doxycycline
Observed In:	hepatocyte apoptotic body hepatocyte apoptotic process liver
Stage:	Days 7-13

Phenotype Detail

Acknowledgments

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Figure 5

Figure 5

ahctf1 heterozygosity amplifies cell death in dox-treated TO(krasG12V)T/+ hepatocytes in the presence and absence of Tp53.

ahctf1 heterozygosity amplifies cell death in dox-treated TO(kras^G12V)^T/+ hepatocytes in the presence and absence of Tp53.