FIGURE

Fig. 5

ID

ZDB-FIG-230122-15

Publication

Gong et al., 2023 - Hyperaminoacidemia induces pancreatic α cell proliferation via synergism between the mTORC1 and CaSR-Gq signaling pathways

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Fig. 5

Activation of a Gq-DREADD in α cells restores proliferation in casr−/−;gcgr^DKO zebrafish.

a Diagram of CNO treatment, 20 μM for 48 h. b Representative images of α cells in control, Tgα^Gq(Tg(gcga:hM3Dq, cryaa:tagRFP));casr−/−;gcgr^DKO, and Tgα^Gq;gcgr^DKO larvae with 20 μM CNO or vehicle at 5 dpf (scale bar, 10 μm). c Quantification of α cell numbers at 5 dpf in control, Tgα^Gq;casr−/−;gcgr^DKO, and Tgα^Gq;gcgr^DKO larvae treated with 20 μM CNO or vehicle for 48 h (data represent the means ± SD, n = 12 for each group). d Representative images of EdU staining at 5 dpf in control, Tgα^Gq;casr−/−;gcgr^DKO and Tgα^Gq; gcgr^DKO larvae treated with 20 μM CNO or vehicle for 48 h. EdU (red)-positive α cells are indicated by arrows (scale bar, 10 μm). e Quantification of EdU-positive α cell numbers at 5 dpf in control, Tgα^Gq;casr−/−;gcgr^DKO, and Tgα^Gq;gcgr^DKO larvae treated with 20 μM CNO or vehicle for 48 h (data represent the means ± SD, n = 8 for each group). ***P < 0.001, ****P < 0.0001, NS indicates no significant difference (One-way ANOVA, Tukey’s multiple comparisons test, the quantifications represent individual islet sections). Source data are provided as a ^{Source Data} file.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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