FIGURE 6

Reduced adiposity in Smc5^K371/K371 mice. (A) Glucose levels (assessed by an intraperitoneal glucose tolerance test) at 14 weeks of age were significantly higher in K371/K371 mice than in wild‐type (WT) control mice at the 30‐min time point. (B) In an insulin tolerance test, glucose was reduced to a lesser extent trend in K371/K371 mice than in their WT control counterparts at 12 weeks of age. Pairwise significance compared to controls is shown. In parts (A and B), n = 3 for each group the analysis used two‐way ANOVA with repeated measures and Bonferroni correction (GraphPad Prism 6). All error bars are plotted as a mean value ± s.e.m. (C) Representative two‐dimensional cross‐sectional μCT images in transverse planes of the abdomen (top), scapular (middle) and coronal sections of the whole body (bottom) showing white and brown adipose tissue. Scale bars = 10 mm. The fat volume was quantified and shown in the right panel. (D and E) Histological analysis of E18.5 embryos. Sagittal sections of the cervical/thoracic area were stained with haematoxylin and eosin (H&E) (D) or with antibodies against the brown adipose (B) marker UCP1 (green) and the muscle (M) marker Myosin (red) (E). Scale bar = 400 μm. Boxed regions in each image were magnified and showed details of embryonic brown adipose precursors. Lipid droplets can be seen forming immaturely in the mutants. (F) Oil red O staining of WT and mutant mouse embryonic fibroblasts (MEFs) after 7 days of white adipogenesis. Scale bar = 100 μm