FIGURE 1

Experimental overview and statistical approach: (a): (i) WT stock assigned to obesogenic and control groups at 12 weeks post-fertilization (wpf); (ii) designated feeding amounts of Artemia; (iii) body weight measurements began at 14 wpf (continued every 2 weeks until the end of the experiment at 36 wpf); (iv) maintenance breeding within tanks occurred every 2–3 weeks for general health purposes and to prevent females from becoming eggbound (v) behavioral assays began at 24 wpf (3-week intervals); (vi) aversive learning assay began; (vii) final body weight measurement and fish were sacrificed for FBG (fasting blood glucose) measurements; (b) Statistical approaches: mean and variance differences calculated between control and obesogenic diet zebrafish groups through the use of mixed models; repeatability of behavior estimates calculated as the proportion of between-group (between-individual) variance out of total variance; and estimating whether different personality traits correlate at the between-individual level of variation (known as “behavioral syndromes”).