FIGURE

Fig. 1

ID

ZDB-FIG-221209-20

Publication

Moss et al., 2021 - Autophagy coordinates chondrocyte development and early joint formation in zebrafish

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Fig. 1

Generation of atg13 knock out zebrafish line. (A) Schematic showing location of 5 bp deletion in atg13 knockout line and generation of premature STOP codon, with black lines representing exons. Key domains within atg13 highlighted; LIR, Lc3‐interacting region; MIM, Microtubule interacting motif. (B) Immunoblot showing loss of atg13 expression in atg13 homozygous mutant. (C) Lateral widefield images of atg13 zebrafish larvae at 5 dpf. Orange arrowheads indicate phenotypic differences in development between wt and atg13 mutants (from left to right: bent body axis, uninflated swim bladder and shorter body length). Scale bar = 500 µm. (D) Graph showing whole body length of atg13 larvae from 1–5 dpf. Student's t‐test performed at 5 dpf between wt and atg13 mutant, *p = .0013. (E) Kaplan–Meier graph showing survival of larvae up to 20 dpf

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	atg13^{fci500/fci500} atg13^fci500/+
Observed In:	swim bladder trunk whole organism
Stage Range:	Prim-5 to Days 14-20

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ FASEB J.