FIGURE

Fig. 7

ID

ZDB-FIG-221119-33

Publication

Coffey et al., 2021 - Lysosomal Function Impacts the Skeletal Muscle Extracellular Matrix

Other Figures

All Figure Page

Back to All Figure Page

Fig. 7

mmp9 mRNA and protein are upregulated in spns1^−/− larvae at 3.5 dpf. (A,B) Anterior left, dorsal top, side-mounted larvae. Phalloidin (white) to visualize actin and MMP-9 antibody staining (pseudo-colored fuchsia). (A,A2,B,B2) Merged phalloidin and MMP-9 channels and (A1,B1,A3,B3) MMP-9 single channel. (A1) MMP-9 is not detected in WT muscle. (B1) MMP-9 is detected in spns1^−/− muscle. White arrowheads highlight MMP-9 staining (or lack thereof in wild-type larvae). (C) Relative mmp9 mRNA expression in WT and spns1^−/− larvae measured as fold change in 3.5 dpf larvae. (D) Percentage of spns1^−/− and WT at 1 and 3.5 dpf with qualitatively scored relative fluorescence intensity. ** p < 0.01, **** p < 0.0001. Scale bars 50 µm.

Expression Data

Gene:	mmp9
Fish:	WT spns1^{hi891Tg/hi891Tg}
Anatomical Terms:	myotome skeletal muscle cell whole organism
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	spns1^{hi891Tg/hi891Tg}
Observed In:	myotome skeletal muscle cell whole organism
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ J Dev Biol