Measurement of hypoalbuminemia-like phenotype in nphs1-/- zebrafish. (A,C,E) The GFP fluorescence in wild-type siblings with Tg (l-fabp:VDBP-EGFP) at 7-9 dpf. (B,D,F) The GFP fluorescence in nphs1-/- mutants with Tg (l-fabp:VDBP-EGFP) at 7-9 dpf. (G–J) The GFP fluorescence in retina vessels and dorsal aorta of wild type fish (G,I) and of nphs1-/- mutants (H,J) at 8 dpf. (K) The quantitative measurements of the GFP fluorescence intensity in the retinal vessels (G,H) and the dorsal aorta (I–J) (arrow) using ImageJ. The error bars show the standard error of the mean (SEM) (*p ≤ 0. 1, ****p ≤ 0. 0001) (Student’s t-test).
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