FIGURE

Fig. 5

ID

ZDB-FIG-220927-90

Publication

Naylor et al., 2022 - A novel nanoluciferase transgenic reporter measures proteinuria in zebrafish

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Fig. 5

Figure 5. Depletion of col4a3 or col4a4 induces Alport syndrome phenotypes in zebrafish. (a) Bar charts showing real-time reverse transcription–polymerase chain reaction analysis of col4a3 and col4a4 in wild-type versus respective crispant embryos (n = 3). (b) Transmission electron microscopy images of the glomerular filtration barrier in control scr, col4a3, and col4a4 crispants (bar = 500 nm). Podocytes are pseudocolored green, the glomerular basement membrane (GBM) is yellow, and the endothelium is red. (c) Box and whisker plot showing the average GBM width in control scr, col4a3, and col4a4 crispants. (d) Box and whisker plot showing the average foot process width in control scr, col4a3, and col4a4 crispants.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + CRISPR1-col4a3 + CRISPR2-col4a3 + CRISPR3-col4a3 + CRISPR4-col4a3 WT + CRISPR1-col4a4 + CRISPR2-col4a4 + CRISPR3-col4a4 + CRISPR4-col4a4
Knockdown Reagents:	CRISPR1-col4a3 CRISPR1-col4a4 CRISPR2-col4a3 CRISPR2-col4a4 CRISPR3-col4a3 CRISPR3-col4a4 CRISPR4-col4a3 CRISPR4-col4a4
Observed In:	pronephric glomerular basement membrane pronephric podocyte podocyte foot
Stage:	Day 4

Phenotype Detail

Acknowledgments

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