FIGURE

Fig. 4

ID

ZDB-FIG-220905-43

Publication

Iyer et al., 2022 - A lysosomal regulatory circuit essential for the development and function of microglia

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Fig. 4

Fig. 4. Microglia numbers are restored in rraga mutants with simultaneous mutation of tfeb, tfe3a, or tfe3b.

Images show dorsal views of the midbrain (anterior on top) of neutral red (NR)–stained larvae of indicated genotypes at 5 dpf, and graphs show corresponding quantification. (A and D) Progeny from rraga^+/−; tfeb^+/− intercross, (B and E) Progeny from rraga^+/−; tfe3a^+/− intercross. (C and F) Progeny from rraga^+/−; tfe3b^+/− intercross. (G to I) Progeny from rraga^+/−; tfeb^+/−; tfe3a^+/−; tfe3b^+/− quadruple heterozygous intercross. Images of a rraga mutant and tfeb and tfe3 genotypes that result in complete rescue of NR⁺ microglia in rraga mutants are shown in (H). In (G), the same genotypes, but in a background wild-type for rraga, are included for comparison. All graphs show mean + SD; significance was determined using parametric unpaired t test. The number of animals analyzed for each experiment is listed in the tables below each graph; (A) to (F) are representative of at least two independent experiments.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	rraga^st77/st77 rraga^st77/+; tfe3a^st124/+ rraga^st77/+; tfe3a^st124/st124 rraga^st77/+; tfe3b^st125/+ rraga^st77/+; tfe3b^st125/st125 rraga^st77/+; tfeb^st120/+ rraga^st77/+; tfeb^st120/st120 rraga^st77/st77; tfe3a^st124/+ rraga^st77/st77; tfe3a^st124/st124 rraga^st77/st77; tfe3b^st125/+ rraga^st77/st77; tfe3b^st125/st125 rraga^st77/st77; tfeb^st120/+ rraga^st77/st77; tfeb^st120/st120 rraga^st77/st77; tfe3a^st124/+; tfe3b^st125/+; tfeb^st120/+
Observed In:	midbrain microglial cell
Stage:	Day 5

Phenotype Detail

Acknowledgments

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