Fig. 1

LGP2 deficiency impairs zebrafish survival against SVCV infection

(A and B) RT-PCR detection of lgp2 (or lgp2-talen) mRNA in the indicated tissues from lgp2^+/+ and lgp2^lof/lof zebrafish adults (60 dpf) in healthy state (A) or following i.p. injection with SVCV (10⁸ TCID₅₀/mL) (B).

(C) Western blot analysis of LGP2 protein in the gill from lgp2^+/+ and lgp2^lof/lof zebrafish (120 dpf) by with LGP2 antibody. ∗ indicated the LGP2 protein band. The bands below ∗ were nonspecific proteins.

(D) Representative imaging of lgp2^+/+ and lgp2^lof/lof zebrafish larvae (6 dpf, n = 50) immersed with SVCV (2×10⁶ TCID₅₀/mL) for 2 d. Red arrows indicated the dead larvae. (E and F) Mortality analysis of lgp2^lof/lof larvae (E) and adults (F) together with WT zebrafish following SVCV infection.

(E) lgp2^+/+ and lgp2^lof/lof zebrafish larvae (6 dpf, n = 100) were immersed with SVCV (5×10⁵ TCID₅₀/mL).

(F) lgp2^+/+ and lgp2^lof/lof zebrafish adults (60 dpf, n = 50) were injected i.p. with SVCV (10⁸ TCID₅₀/mL), at 20 μL per fish.

(G and H) LGP2 deficiency exacerbated SVCV replication in zebrafish larvae (G) and adults (H). lgp2^lof/lof larvae (6 dpf) and adults (60 dpf) together with WT zebrafish were infected with SVCV as in (E) and (F), respectively. The expression of five SVCV genes, including nucleoprotein (N), phosphoprotein (P), matrix protein (M), glycoprotein (G), and RNA polymerase (L) was detected by RT-PCR. Data were shown as mean ± SD (N = 3). P values were calculated using Student’s t test. ∗p < 0.05, ∗∗p < 0.01. See also Figure S1, and Table S1.