FIGURE

Fig. 3

ID

ZDB-FIG-220810-19

Publication

Chen et al., 2022 - Leukocyte invasion of the brain after peripheral trauma in zebrafish (Danio rerio)

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Fig. 3

a Experimental setup. b Schematic showing antisense morpholino (MO)-mediated transient knockdown. c, d Light-sheet imaging showed a significantly decreased number of pu.1 + myeloid cells by pu.1 MO knockdown in the zebrafish brain at 3 dpf. Independent t test, **, p < 0.01. Scale bar, 10 µm. e, f Light-sheet imaging showed that pu.1 MO knockdown inhibited the increase in pu.1 + myeloid cells in the zebrafish brain at 1 dpt. Two-way ANOVA with Tukey’s HSD post hoc test, **, p < 0.01 compared with CTRL. Scale bar, 20 µm. g, h Schematic showing the locomotor behavioral assay and thigmotaxis for zebrafish larvae. i Peripheral trauma increased the swim speed and active time of zebrafish larvae at 7 dpf/4 dpt. Independent t test, **, p < 0.01. j–k Behavioral assays suggested anxiety-like behaviors, including hyperactivity and thigmotaxis, in zebrafish at 4 dpi, which could be rescued by pu.1 MO knockdown. Two-way ANOVA with Tukey’s HSD post hoc test, *, p < 0.05 compared with CTRL. l–m il1b mutation rescued hyperactivity but not thigmotaxis at 4 dpi. Two-way ANOVA with Tukey’s HSD post hoc test, *, p < 0.05 compared to CTRL, #, p < 0.05 compared to il1b^Mut.

Expression Data

Gene:	EGFP
Fish:	zdf11Tg zdf11Tg + MO1-spi1b
Knockdown Reagent:	MO1-spi1b
Anatomical Term:	brain myeloid cell
Stage:	Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT WT + MO1-spi1b zdf11Tg zdf11Tg + MO1-spi1b
Condition:	amputation: caudal fin
Knockdown Reagent:	MO1-spi1b
Observed In:	brain myeloid cell midbrain myeloid cell swimming
Stage Range:	Protruding-mouth to Days 7-13

Phenotype Detail

Acknowledgments

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