Fig. 4
- ID
- ZDB-FIG-220718-20
- Publication
- Li et al., 2022 - Estrogens revert neutrophil hyperplasia by inhibiting Hif1α-cMyb pathway in zebrafish myelodysplastic syndromes models
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E2 decreases c-myb expression in c-mybhyper transgenic zebrafish embryos.
A E2 exposure decreased c-myb in the CHT, as determined by WISH. B qPCR quantification of the decreased c-myb expression by E2 (the c-mybhyper fusion gene contained two parts, which comprised truncated c-myb from exon1 to exon10, followed by a near full-length c-myb from exon2 to exon 15). c-myb-primer1 and c-myb-primer2 were designed at repetitive and nonrepetitive sequences. (t-test, mean ± SEM. ***p < 0.001, **p < 0.01, n > 10). C qPCR quantification of decreased c-myb expression in c-mybhyper adult zebrafish kidney after treatment with E2. (t-test, mean ± SEM. ***p < 0.001, *p < 0.05, n > 10) (D) FACS analysis confirmed that E2 diminished c-myb:GFP+ cells. (t-test, mean ± SEM. *p < 0.05, n > 10) (E) qPCR quantification of decreased c-myb expression in c-myb:GFP+ cells by E2. (t-test, mean ± SEM. ***p < 0.001, n > 10). F qPCR quantification of decreased c-myb expression in lyz:Dsred+ cells by E2. (t-test, mean ± SEM. ***p < 0.001, **p < 0.01, n > 10). |