FIGURE

Fig. 2.

ID

ZDB-FIG-220717-108

Publication

Derrick et al., 2022 - Sequential action of jnk genes establishes the embryonic left-right axis

Other Figures

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Fig. 2.

jnk1a and jnk1b are required for fluid flow in KV. (A) Schematic showing KV (magenta) at the base of the notochord (grey) and direction of nodal flow (arrow) at 10-14 ss. The notochord was used to define the antero-posterior and left-right axes for subdivision of KV into quadrants. (B-E) Representative bright-field images of wild type (B), MZjnk1a (C), MZjnk1b (D) and MZjnk1a;MZjnk1b KVs (white dotted line), and traces of bead trajectories over time (coloured lines), together with mean bead speed (µm/s) in each quadrant±s.d. (F) Quantification of average bead speed in KV between 10 and 14 ss. MZjnk1a and MZjnk1b mutant embryos have significantly slower average nodal flow compared with wild-type controls. Loss of both jnk1a and jnk1b results in a greater reduction in average speed. (F) Data are median±interquartile range, Kruskal-Wallis test, multiple comparisons. Wild type, n=22 beads across six embryos; MZjnk1a, n=26 beads across four embryos; MZjnk1b, n=23 beads across three embryos; MZjnk1a;MZjnk1b, n=25 beads across four embryos. (B-E) Anterior is upwards; left is leftwards. ns, not significant. *P<0.05, ***P<0.001, ****P<0.0001. AL, anterior left; AR, anterior right; PL, posterior left; PR, posterior right.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	mapk8a^n2/n2 mapk8b^n3/n3 mapk8a^n2/n2; mapk8b^n3/n3
Observed In:	Kupffer's vesicle epithelial cilium movement involved in extracellular fluid movement Kupffer's vesicle Kupffer's vesicle development
Stage:	10-13 somites

Phenotype Detail

Acknowledgments

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