FIGURE

Fig. 4

ID

ZDB-FIG-220715-66

Publication

Owen et al., 2022 - Identification of 4 novel human ocular coloboma genes ANK3, BMPR1B, PDGFRA, and CDH4 through evolutionary conserved vertebrate gene analysis

Other Figures

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Fig. 4

Figure 4. MO knockdown and rescue experiments of bmpr1ba/b in zebrafish. Injection of the MOs alone showed reduction in eye size and the presence of a coloboma. A. Coinjection with in vitro synthesized human BMPR1B messenger RNA (mRNA) rescues the phenotypic effects of the MO alone. Human variants identified within the Genomics England cohort were expressed and mRNAs coinjected with each MO. Scale bar = 500 μm. Eye diameter was reported for (B) bmpr1ba and (C) bmpr1bb compared with wild-type uninjected; MO; MO + human wild-type BMPR1B; and variants Arg91Ile, Arg224His, Arg224Leu, and Arg376Glu. (n for bmpr1ba experiments = 23, 25, 55, 13, 21, 28, 42 and n for bmpr1bb experiments = 23, 56, 55, 32, 34, 33, 24). Unpaired t tests were used to compare data. ∗∗P < .01, ∗∗∗P < .001, ∗∗∗∗P < .0001, ns P > .05. MO, morpholino; ns, nonsignificant.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO4-bmpr1ba AB + MO5-bmpr1bb
Knockdown Reagents:	MO4-bmpr1ba MO5-bmpr1bb
Observed In:	eye
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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