Fig. 1

Main expression domains of the PDGFRβ-gal4:UAS-EGFP line as seen by high-content confocal imaging of 21 dpf juveniles. Three main meningeal domains were segmented and color-coded in orange, yellow and blue. (A,B). 3D reconstructions using the AMIRA software. Midline roof cells (MID): orange; arachnoid cells (ARA): yellow; red blood cells (RBC): blue; vessels: magenta. (A). Dorsal view. White rectangles indicate locations of details in (C–E). (B). Lateral view. Orange arrowheads show the flat MID. (C). XZ thick optical section showing one pericyte (yellow arrowhead) and one vessel (white arrow). (D). Dorsal view of choroid plexus. (E). Red Blood Cells (RBC). (F). Dorsal view of ARA showing typical elongated and square shapes (as described for mammalian interstitial arachnoid cells) and granulations (yellow arrowheads). (G). Dorsal view of MID stained with Aqp1a.1 antibody showing their round and flat ameboid-like shape. Scale bars: (A,B): 100 μm. (C–G): 50 μm. (D–E): 10 μm.