FIGURE

Fig. 4

ID

ZDB-FIG-220705-64

Publication

Perl et al., 2022 - Stx4 is required to regulate cardiomyocyte Ca²⁺ handling during vertebrate cardiac development

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Fig. 4

Stx4 regulates Ca²⁺ handling in CMs

(A and B) Screenshots from high-speed images of 72 hpf WT and stx4 mutant larvae showing a ventricular region of interest (ROI) and the resulting kymograph used to quantify heart rates. Scale bars, 100 μM.

(C) Heart rates (HR) determined from ventricular ROIs of 72 hpf WT and stx4 mutant larvae captured by high-speed imaging and quantified as beats per minute (bpm). Mutants exhibit an ∼40% reduction in HR versus WT (WT mean HR: 208.4 bpm, Mutant mean HR: 124.3 bpm). Data are represented as the mean ± SEM, n = 16 larvae/group, Student’s t test, ∗∗∗∗p < 0.0001.

(D and E) Maps of Ca²⁺ transient amplitudes from paced hearts of 72 hpf WT or stx4 mutant larvae. A, atrium; V, ventricle. The color scale depicts Ca²⁺ transient amplitudes in fluorescence ratio units (F340/F380).

(F and G) Ca²⁺ transient amplitudes from atrial or ventricular ROIs, respectively.

(H and I) Ca²⁺ transient durations from atrial and ventricular ROIs. Data are represented as the mean ± SEM, n = 14 WT/stx4^+/− and n = 8 stx4^−/−, Student’s t test, ∗∗p < 0.01.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	stx4^{ci1016/ci1016}
Observed In:	atrial myocardium regulation of cardiac muscle contraction by calcium ion signaling heart contraction ventricular myocardium regulation of cardiac muscle contraction by calcium ion signaling
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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