FIGURE

Fig. 6

ID

ZDB-FIG-220630-15

Publication

Bruton et al., 2022 - Macrophages trigger cardiomyocyte proliferation by increasing epicardial vegfaa expression during larval zebrafish heart regeneration

Other Figures

All Figure Page

Back to All Figure Page

Fig. 6

Figure 6. Macrophages stimulate epicardial cell number expansion following cardiac injury

(A) Image of an uninjured 3 dpf ventricle from a Tg(myl7:mKateCAAX;myl7:h2b-GFP) larva showing vegfaa+ cells (green) overlying myocardium (magenta). Cyan box, zoom panel.

(B) Images of uninjured and injured ventricles from Tg(vegfaa:GFP) larvae acquired at 48 hpi. “Heat” LUT is applied to highlight the increased intensity of epicardial vegfaa:GFP in injured hearts.

(C) Total ventricular vegfaa:GFP fluorescence in uninjured and injured hearts over standard injury model time points, n = 28–30. ^∗ p ≤ 0.05 one-way ANOVA followed by Holms-Sidak’s multiple comparison post-hoc tests.

(D and E) Images of uninjured and injured EdU-stained Tg(vegfaa:GFP) hearts 48 hpi showing EdU+ epicardial cells (white arrowheads) and the proportion of EdU+ epicardial cells are quantified in (E), n = 13–16. Unpaired t test, ^∗∗ p ≤ 0.01.

(F) Image of a ventricle from a Tg(vegfaa:GFP;csfr1a:NfsB-mCherry;kdrl:hsa.HRAS-mCherry) (abbreviated to kdrl:mCherry) larva at 48 hpi showing macrophages in the epicardial-myocardial niche (white arrowheads). Cyan box, zoom panel.

(G) Images of uninjured and injured ventricles from Tg(vegfaa:GFP;csfr1a:NfsB-mCherry) larvae from metronidazole-nitroreductase macrophage ablation groups at 48 hpi. “Heat” LUT is applied to highlight the increase in the overall fluorescence in injured groups except NTR+met+. Total vegfaa:GFP fluorescence (H) and epicardial cell number (I) in uninjured and injured ventricles from Tg(vegfaa:GFP;csfr1a:NfsB-mCherry) larvae from metronidazole-nitroreductase macrophage ablation groups at 48 hpi. All images are maximum intensity projections of 3D LSFM stacks. Scale bars, 50 μm, n = 46. ^∗ p ≤ 0.05, ^∗∗ p ≤ 0.01, ^∗∗∗p ≤ 0.001. One-way ANOVA followed by Holms-Sidak’s multiple comparison post-hoc tests. Data are represented as mean ± SEM.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image.

Reprinted from Developmental Cell, 57(12), Bruton, F.A., Kaveh, A., Ross-Stewart, K.M., Matrone, G., Oremek, M.E.M., Solomonidis, E.G., Tucker, C.S., Mullins, J.J., Lucas, C.D., Brittan, M., Taylor, J.M., Rossi, A.G., Denvir, M.A., Macrophages trigger cardiomyocyte proliferation by increasing epicardial vegfaa expression during larval zebrafish heart regeneration, 1512-1528.e5, Copyright (2022) with permission from Elsevier. Full text @ Dev. Cell