FIGURE

Fig. 1

ID

ZDB-FIG-220628-149

Publication

He et al., 2021 - Zebrafish foxc1a controls ventricular chamber maturation by directly regulating wwtr1 and nkx2.5 expression

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Fig. 1

Fig. 1. Ventricular development defects are observed during the trabecular stage in foxc1a^nju18 mutants. A: Maximum intensity projections of confocal z-stacks show representative ventricles of 60 hpf Tg(myl7:nDsRed);foxc1a^+/+ (n = 11) and Tg(myl7:nDsRed);foxc1a^nju18 (n = 12) embryos. B: Maximum intensity projections of confocal z-stacks show representative hearts of 96 hpf WT (n = 8) and foxc1a^nju18 (n = 10) embryos. C: A scatter plot shows the numbers of cardiomyocytes in the atria and ventricles. D: Midsagittal confocal images show hearts of 80 hpf Tg(myl7:eGFP)^m225;foxc1a^+/+ (n = 14) and Tg(myl7:eGFP)^m225;foxc1a^nju18 (n = 10) embryos. E: Midsagittal confocal images show hearts of 96 hpf Tg(myl7:eGFP)^m225; foxc1a^+/+ (n = 13) and Tg(myl7:eGFP)^m225;foxc1a^nju18 (n = 17) embryos. F: Statistical analysis of data derived from (D) and (E). G: Maximum intensity projections of confocal z-stacks show representative EdU-stained hearts from 84 hpf Tg(myl7:nlsRed);foxc1a^+/? (n = 9) and Tg(myl7:nDsRed);foxc1a^nju18 (n = 10) embryos. H: A scatter plot shows the percentage of EdU⁺ cells. Scale bars, 50 μm. ns, P > 0.05; ∗∗, P < 0.01; ∗∗∗, P < 0.001; ∗∗∗∗, P < 0.0001. Error bars represent the standard deviation. hpf, hour postfertilization.

Expression Data

Genes:	DsRed GFP
Fish:	f1Tg zf3348Tg foxc1a^nju18/nju18; f1Tg foxc1a^nju18/nju18; zf3348Tg
Anatomical Terms:	cardiac muscle cell cardiac muscle cell nucleus
Stage Range:	Pec-fin to Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	foxc1a^nju18/nju18; f1Tg foxc1a^nju18/nju18; zf3348Tg
Observed In:	atrium atrium cell proliferation involved in heart morphogenesis cardiac muscle cell cardiac ventricle cardiac ventricle cell proliferation involved in heart morphogenesis cardiac ventricle ventricular trabecula myocardium morphogenesis trabecular layer of ventricle
Stage Range:	Pec-fin to Day 4

Phenotype Detail

Acknowledgments

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Reprinted from Journal of genetics and genomics = Yi chuan xue bao, 49(6), He, L., Zhang, Q., Jiang, D., Zhang, Y., Wei, Y., Yang, Y., Li, N., Wang, S., Yue, Y., Zhao, Q., Zebrafish foxc1a controls ventricular chamber maturation by directly regulating wwtr1 and nkx2.5 expression, 559-568, Copyright (2021) with permission from Elsevier. Full text @ J. Genet. Genomics