Figure 3. The Dvl and ETTV motifs act in a partially redundant manner during zebrafish brain angiogenesis (A) Scheme of zebrafish Gpr124 highlighting the CRISPR-Cas9 target site corresponding to the gpr124ulb14(ΔETTV) mutant allele. (B) Lateral views of WT and ΔETTV−/− mutant embryos at 60 hpf. (C) Lateral views of the skin-pigmentation patterns of WT and ΔETTV−/− mutant adults. (D) Dorsal views of hindbrain vasculature of WT and ΔETTV−/− mutants. (E) Quantification of CtAs of WT and ΔETTV−/− mutant embryos at 48 hpf. (F) Same as (E) at 60 hpf. (G) 3D representation of cerebrovasculature of 60 hpf WT and ΔETTV mutant embryos. (H) Same as (E) in 5 dpf larvae. (I) Quantification of the number of lumenized CtAs in 48 hpf WT and ΔETTV mutant embryos. (J and K) Dorsal views (J) and quantification (K) of dorsal root ganglia in the trunk region of WT and ΔETTV mutant larvae at 72 hpf. (L) Luciferase activities of HEK293 STF cells co-transfected with Wnt7a, Fz1, Reck, and increasing amounts of the indicated Gpr124 ICD variants; n = 3. (M) Quantification of hindbrain CtAs of gpr124 morphant embryos at 48 hpf injected at the one-cell stage with 100 pg of the indicated mRNAs. Scale bars, 0.5 mm for (B) and 100 μm for (D), (G), and (J). Related to Figure S1.
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