FIGURE
Fig. 4
- ID
- ZDB-FIG-220527-4
- Publication
- Gao et al., 2022 - Accumulation of Lipid Droplets in a Novel Bietti Crystalline Dystrophy Zebrafish Model With Impaired PPARα Pathway
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Fig. 4
Lipid accumulation in RPE cells of WT and cyp4v7−/−cyp4v8−/− zebrafish. (A) Nile red staining of lipid droplets in retinal frozen sections of WT and cyp4v7−/−cyp4v8−/− zebrafish at 3, 7, 10, and 12 mpf. White arrows, lipid droplets. Scale bar: 20 µm. (B) Nile red staining of RPE flat mounts from WT and cyp4v7−/−cyp4v8−/− zebrafish at 3, 7, and 10 months after fertilization. White arrows, lipid droplets (bright red). Scale bar: 20 µm. Bar graphs show LD size distribution in cyp4v7−/−cyp4v8−/− zebrafish RPE flat mounts at 3, 7, and 10 months after fertilization. Diameter of LDs in cyp4v7−/−cyp4v8−/− zebrafish RPE cell was measured and represented by white (>3 μm), black (2-3 μm), red (1-2 μm), and blue (0-1 μm) (n = 88-103 RPE cells examined over three biologically independent experiments). (C) TEM images of retinal ultrathin sections from WT and cyp4v7−/−cyp4v8−/− zebrafish at seven months after fertilization. Lipid droplets are labeled with red text “LD”. Vacuoles are labeled with red text “V.”
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Expression Data
Expression Detail
Antibody Labeling
Phenotype Data
| Fish: | |
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| Observed In: | |
| Stage: | Adult |
Phenotype Detail
Acknowledgments
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Full text @ Invest. Ophthalmol. Vis. Sci.