Figure EV3

Overview of visual motor response (VMR) data

A–C   Actograms show the mean activity of wild‐type (dark blue line) and opn4 dko (grey line) under a medium intensity LD 12:12 h regime at the light to dark boundary on the (A) 5^th dpf, (B) 6^th dpf and (C) 7^th dpf. O‐bend spike indicated with red arrowhead. The bar under the chart indicates the last 6 min of the light phase and the first 12 min of the dark phase. Solely for estimation, the mean activity over the last 6 min in the light phase is plotted as a horizontal line for wild‐type (green) and opn4 dko (red).

D–F   Actogram shows mean activity of wild‐type larvae under a medium (dark blue line) or low (light blue line) intensity LD 12:12 h regime at the light to dark boundary on the (D) 5^th dpf, (E) 6^th dpf and (F) 7^th dpf. Solely for estimation, the mean activity over the last 6 min in the light phase is plotted as a horizontal line for larvae under medium light intensity (green) and low light intensity (red).

G   opn4 dko larvae (grey box) show similar displacement in routine turns after transition from light to darkness as wild‐type (blue box) on the 6^th and 7^th dpf, but not on the 5^th dpf (U‐test, P = 0.0413). Note that displacement of each individual larva was calculated separately by comparison with its own baseline activity in the light phase. Thus the displacement of each larva on one particular day is in this case one single observation in the test statistics.

H   Wild‐type larvae show similar displacement in routine turns after transition from light to darkness under a low (light blue box) as under a medium (dark blue box) intensity LD regime on the 6^th and 7^th dpf, but not on the 5^th dpf (U‐test, P = 0.0029).

I   Plot of the photon flux at medium (dark blue line) and low (light blue line) light intensities. For biological processes, the photon flux has a higher relevance than the irradiance (power of electromagnetic radiation) because a photoreceptor is activated by a photon. The photon flux is defined as the number of photons (γ) per unit area (m²/s).

Data information: In (G) and (H), boxplot divides the data in quartiles: the box indicates the interquartile range, with the horizontal line in the box denoting the median of the data set, the whiskers extend to the minimum and maximum, and meet the box at the median of the lower (quartile 1) and median of the upper (quartile 3) half of the dataset. Black dots indicate biological replicates (n = 18), the red dot indicates the mean, red error bars indicate the 95% confidence interval and asterisks indicate significance (0.001 < P(**) < 0.01, P(***) < 0.001, ns = not significant).