FIGURE

Figure 4

ID

ZDB-FIG-220426-82

Publication

Lane et al., 2021 - Steroid-sensitive nephrotic syndrome candidate gene CLVS1 regulates podocyte oxidative stress and endocytosis

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Figure 4

Loss of CLVS1 causes decreased podocyte endocytosis.

(A–C) Images showing the decreased endocytosis of fluorescently labeled dextran (pHrodo Dextran) in conditionally immortalized podocytes with CRISPR/Cas9-mediated KO of CLVS1 that can be restored with treatment with 1 μM dexamethasone (representative molecules circled in red). (D) Quantification of these images revealed a significant (P = 0.0193) loss of dextran endocytosis in KO podocytes compared with controls that was eliminated when these podocytes were treated with dexamethasone (P = 0.7568, n > 20 for each experimental group, 1-way ANOVA). A selective inhibitor of dynamin I and dynamin II, Dynasore, which reduces clathrin-mediated endocytosis, was used as an endocytosis control. Errors bars depict SEM.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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