FIGURE

Figure 3

ID

ZDB-FIG-220402-28

Publication

Kulkarni et al., 2022 - A Novel 2-Hit Zebrafish Model to Study Early Pathogenesis of Non-Alcoholic Fatty Liver Disease

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Figure 3

Macrophage infiltration is increased in HFD, Li-PIC3, and Li-PIC3+HFD larvae. (A–D) 7 dpf larvae labeled with GFP immunostaining to reveal macrophages and either a 2CLIP transgene (A,B) or immunolabeled with Prox1 antibody (C,D) to reveal hepatocytes. Infiltrating macrophages are indicated by white arrows. (E) Quantification of macrophages within the liver boundary reveals a statistically significant increase with HFD and Li-PIC3+HFD treatments. Statistical analysis by one-way ANOVA, ns-non significant, ** p < 0.01, *** p < 0.001.

Expression Data

Genes:	DsRed EGFP
Antibody:	Ab14-prox1
Fish:	gl22Tg gl22Tg; gz15Tg ch407Tg; gl22Tg; iu15Tg
Conditions:	chemical treatment by environment: doxycycline chemical treatment by environment: doxycycline, high fat high fat
Anatomical Terms:	hepatocyte liver macrophage
Stage:	Days 7-13

Expression Detail

Gene	Fish	Conditions	Stage	Anatomy	Assay
DsRed	gl22Tg	high fat	Days 7-13	hepatocyte	IFL
DsRed	gl22Tg; gz15Tg	high fat	Days 7-13	hepatocyte	IFL
EGFP	gl22Tg	high fat	Days 7-13	liver macrophage	IFL
EGFP	gl22Tg; gz15Tg	high fat	Days 7-13	liver macrophage	IFL

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Antibody Labeling

Phenotype Data

Fish:	gl22Tg gl22Tg; gz15Tg ch407Tg; gl22Tg; iu15Tg
Conditions:	chemical treatment by environment: doxycycline chemical treatment by environment: doxycycline, high fat high fat
Observed In:	liver macrophage
Stage:	Days 7-13

Phenotype Detail

Acknowledgments

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