FIGURE

Fig 1

ID

ZDB-FIG-220319-50

Publication

Liu et al., 2022 - Rod genesis driven by mafba in an nrl knockout zebrafish model with altered photoreceptor composition and progressive retinal degeneration

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Fig 1

Knocking out <italic toggle='yes'>nrl</italic> diminishes but does not eradicate the rod population in zebrafish.

(A) The gene structure of zebrafish nrl and the CRISPR-Cas9 target site used for knocking out nrl are shown. Orange boxes, the exons encoding the MTD and bZIP domains; red arrow, the CRISPR-Cas9 target site; black arrows, the primers used for mutation detection. (B) Sequencing validation of the homozygous nrl del8 mutation (c.230_237del8). (C) The nrl mRNA levels in the nrl-KO zebrafish were measured using qPCR. The data are shown as mean with SD (n = 3). *, p < 0.05. (D) Immunostaining of retinal sections from WT and nrl-KO zebrafish from 1 mpf to 18 mpf with the anti-Rho antibody for the rod outer segments. Scale bars: 25 μm. ROS, rod outer segment; CN, cone nuclear layer; RN, rod nuclear layer; INL, inner nuclear layer; GCL: ganglion cell layer.

Expression Data

Genes:	nrl rho
Antibody:	Ab10-rho
Fish:	WT nrl^hzu16/hzu16
Anatomical Terms:	eye retinal rod cell
Stage Range:	Days 30-44 to Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	nrl^hzu16/hzu16
Observed In:	eye photoreceptor outer segment layer retinal rod cell
Stage Range:	Days 30-44 to Adult

Phenotype Detail

Acknowledgments

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