Fig. 5

GLA-mutant zebrafish show compromised filtration barrier function. (A) Renal impairment is documented by double plasma creatinine level in the mutant zebrafish. (B and D) FPW quantitation in adult zebrafish kidney on TEM images reveals wider foot processes (podocyte foot process effacement) in the mutant (Mann-Whitney Test, P < 0.05. Violin plots depict results within the 95th percentile. The yellow line highlights the GBM. White arrows point to the silt diaphragm between two adjacent foot processes. CL = capillary lumen, F = fenestrae, EC = endothelial cell, US = urinary space, PFP = podocyte foot process. (C) Renal function impairment is further validated by the leakage of high molecular weight proteins observed by SDS-PAGE electrophoresis. The illustrated protein bands in the fig. (80 kDa, 98 kDa and 150 kDa wight arrowheads) were identified by mass spectrometry analysis. MW = marker in kDa, WT = wild type, Mu = Mutant, C = control (water). The polyacrylamide gel image is edited for illustration, full gel image can be reviewed in Sup.7.