FIGURE

Figure 8.

ID

ZDB-FIG-220211-29

Publication

Carril Pardo et al., 2022 - A δ-cell subpopulation with pro-β cell identity contributes to efficient age-independent recovery in a zebrafish diabetes model

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Figure 8.

Protracted bihormonal cells 4 months after β-cell ablation.

(A) Whole mount immunodetection of Ucn3 (red), GFP (green), Ins (grey) on the main islet of Tg(sst1.1:eGFP); Tg(ins:NTR-P2A-mCherry) adult fish revealing persistent bihormonal GFP+ Ins + cells still 4 months after ablation. These cells still also express Ucn3 (white arrowheads). (B–D) Quantification by flow cytometry of islet cell populations in CTL and 4 months after ablation. (B) mCherry+ GFP + bihormonal cells. (C) mCherry+ GFP- monohormonal β-cells. Means ± SD; Unpaired t-test with Welch’s correction; *p < 0.05.

Expression Data

Gene:	EGFP
Antibodies:	Ab1-ins Ab1-ucn3l
Fish:	ulg034Tg/ulg034Tg; ulg054Tg/ulg054Tg
Condition:	chemical treatment by environment: nifurpirinol
Anatomical Term:	pancreatic B cell insulin secreting cell
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	ulg034Tg/ulg034Tg; ulg054Tg/ulg054Tg
Condition:	chemical treatment by environment: nifurpirinol
Observed In:	pancreatic B cell insulin secreting cell
Stage:	Adult

Phenotype Detail

Acknowledgments

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