FIGURE

Figure 3

ID

ZDB-FIG-220131-508

Publication

Prendergast et al., 2022 - Phenotyping Zebrafish Mutant Models to Assess Candidate Genes Associated with Aortic Aneurysm

Other Figures

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Figure 3

Knocking out Ehlers-Danlos and Marfan Syndrome-related genes generates vascular phenotypes. (A) Brightfield macroscopic image of representative series of uninjected larvae at 3 dpf. Scale bar: 1 mm. (B) Similar image of fbn1 knockout fish. Filled arrowhead indicates cardiomegaly. (C) Similar image of col1a2 knockout fish. (D) Similar image of col5a1 knockout fish. (E) Similar image of col5a2a/b knockout fish. (F) Closeup brightfield image of a 6 dpf col5a1 knockout fish with visible aortic hemorrhage (filled arrowhead, scale bar: 500 µm). (G) Confocal micrograph of trunk vasculature of a 4 dpf uninjected Tg(kdr:gfp) fish. DA: dorsal aorta, ISV: intersegmental vessel. Scale bar: 100 µm. (H) Similar micrograph of a col5a1 knockout fish. Empty arrowheads indicate b: excessively branching segment and m: missing segments. (I) Quantification of DA diameter. Diameter is not significantly changed across conditions (t-test, p = 0.998). (J) Quantification of ISV diameter. Diameter is not significantly changed across conditions (t-test, p = 0.342).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + CRISPR3-col1a2 + CRISPR4-col1a2 AB + CRISPR3-col5a1 + CRISPR4-col5a1 AB + CRISPR6-fbn1 + CRISPR7-fbn1 zn1Tg + CRISPR3-col5a1 + CRISPR4-col5a1 AB + CRISPR1-col5a2a + CRISPR1-col5a2b + CRISPR2-col5a2a + CRISPR2-col5a2b
Knockdown Reagents:	CRISPR1-col5a2a CRISPR1-col5a2b CRISPR2-col5a2a CRISPR2-col5a2b CRISPR3-col1a2 CRISPR3-col5a1 CRISPR4-col1a2 CRISPR4-col5a1 CRISPR6-fbn1 CRISPR7-fbn1
Observed In:	dorsal aorta intersegmental vessel pericardium
Stage Range:	Protruding-mouth to Day 6

Phenotype Detail

Acknowledgments

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