Fig. 2

Clearance of small anionic NPs occurs mainly through Stabilin-1. (A) Schematics showing the site of injection within a 56 h post fertilization (hpf) wt or Stabilin knockout zebrafish. In a box, the caudal vein region, where SECs expressing Stabilins are located, a representation of NP circulating or cleared by SECs and (B) representative graph comparing intensity of fluorescent NPs in wt and stab mutants. (C-D) Tissue level view and quantification of fluorescently labeled Qdots. (E-F) CCMV-VLP (G-H) SCNPs. After i.v. injection (1 nL) in wt (AB/TL), stab1ibl3, stab2ibl2 single mutants and stab1ibl3stab2ibl2 double mutants at 1-1.5 hpi. Scale bar: 50 μm. Bar height represents median values, dots represent individual data points, and brackets indicate significant values (*P ˂ 0.05, **P ˂ 0.01, ***P ˂ 0.001) based on Kruskal–Wallis tests followed by two-tailed Dunn’s tests with Bonferroni correction.