FIGURE

Figure 1

ID

ZDB-FIG-211216-163

Publication

Shan et al., 2021 - Pediococcus pentosaceus Enhances Host Resistance Against Pathogen by Increasing IL-1β Production: Understanding Probiotic Effectiveness and Administration Duration

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Figure 1

Administration of the isolated bacteria YC and its protective effect on the resistance against A. hydrophila of zebrafish. (A) Phylogenetic tree of the isolated bacteria bacterium. Distance was calculated by using 16S rRNA gene based on the neighbor-joining criterion and the bootstrap confidence values were 100 replicates. (B) Confocal fluorescence imaging of zebrafish larvae (4 dpf) immersed in water containing 10⁶ CFU/ml P. pentosaceus YC (pre-treated with 300 µM TAMRA) for 24 h. (C) Experimental design for accessing the protective effect of P. pentosaceus YC administration. YC was administrated into water at a concentration of 10⁶ CFU/ml for 1 week (YC1W), 2 weeks (YC2W), 3 weeks (YC3W), and 4 weeks (YC4W). PBS treatment as control group (CON). YC or PBS were provided every 24 h for different durations. (D) Survival curve of zebrafish from different groups following 10⁸ CFU/ml A. hydrophila challenge. Each curve represents the sum of three independent tanks. ***p < 0.001 by Mantel–Cox test.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front Immunol