FIGURE

Figure 2

ID

ZDB-FIG-211207-39

Publication

Min et al., 2021 - ASB2 is a novel E3 ligase of SMAD9 required for cardiogenesis

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Figure 2

ASB2 specifically ubiquitylates SMAD9. HEK 293T cells were co-transfected with vectors coding HA-Ub, Myc-tagged E3 (Myc-ASB2, SMURF1 and SMURF2) and Flag-tagged R-SMADs (Flag-SMAD2, 3, 1, 5, 9) as indicated. Flag-immunoprecipitates or lysates were separated with SDS-PAGE and immunoblotted with the indicated antibodies. The cropped gel images were delineated and uncropped gel images are shown in the Supplementary Information.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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