FIGURE

Figure 7

ID

ZDB-FIG-211120-74

Publication

Mo et al., 2021 - Proper Balance of Small GTPase rab10 Is Critical for PGC Migration in Zebrafish

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Figure 7

rab10 siRNA and cdc42se1 MO failed to rescue mislocalized PGCs in embryos caused by maternal absence of miR-202-5p. (A) Representative images of PGCs in WT embryos injected with 300 pg con siRNA; (A) Representative images of PGCs in Mmir-202 embryos injected with 300 pg con siRNA (B), 50 pg rab10 siRNA (C), 150 pg rab10 siRNA (D), 300 pg rab10 siRNA (E), and 300 pg rab10 siRNA + 4000 pg cdc42se1 MO (E). Arrowheads indicate mislocalized PGCs. (G) The percentage of embryos with mislocalized PGCs with (A–F). The genotypes of the embryos were shown upper right in each panel. (H) The number of mislocalized PGCs in each embryo in the experiment presented in (A–F). (G,H) The results were representative of more than three independent experiments in triplicate. The average number of mislocalized PGCs were showed in red line by mean ± sd. * p < 0.05, ** p < 0.01, **** p < 0.0001. Scale bar, 100 µm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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