FIGURE

Figure 1

ID

ZDB-FIG-211120-109

Publication

Jeong et al., 2021 - Differential Clearance of Aβ Species from the Brain by Brain Lymphatic Endothelial Cells in Zebrafish

Other Figures

All Figure Page

Back to All Figure Page

Figure 1

Monomeric Aβ42 peptides are more efficiently cleared from the brain than oligomeric Aβ42. (A) A schematic diagram of experimental setup. Fluorescently labeled Aβ42 prepared at different time points were injected into the brain followed by in vivo imaging at 5 hpi and 24 hpi. (B,C′) Distribution of injected, fluorescently labeled Aβ42 (mAβ42 or oAβ42) in the brains of 3 dpf larvae at 5 hpi (B,C) and 24 hpi (B′,C′) (left, overlays with brightfield; middle, HiLyte Fluor 555; right, thresholded images of Aβ42 fluorescence). (D,E) Quantification of the area fraction (%) occupied by Aβ42 fluorescence within square unit (512 × 512 pixels) at different time points. (F) Clearance rate of mAβ42 and oAβ42 between 5 hpi and 24 hpi by fluorescence quantification. Two-tailed unpaired t-test, p = 0.023. Data are presented as mean ± SEM. N = 7 per group. Data are representative of at least three independent experiments. dpf, days post fertilization; hpi, hours post injection; HB, hindbrain; OT, optic tectum, Scale bars = 100 μm. * p < 0.05.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Int. J. Mol. Sci.