FIGURE

Fig 2

ID

ZDB-FIG-211116-11

Publication

Bühler et al., 2021 - Histone deacetylase 1 controls cardiomyocyte proliferation during embryonic heart development and cardiac regeneration in zebrafish

Other Figures

All Figure Page

Back to All Figure Page

Fig 2

Early progenitor and chamber specification proceed normally in bal mutants.

(A-D) Whole-mount antisense RNA in situ hybridization (ISH) of cmlc1 and vmhc showed similar expression in bal mutants and wt siblings. (E, F) TUNEL staining (green) and IF for Mef2 (red) on dissected wt (E) and bal mutant hearts (F). (G) Quantification of TUNEL⁺ CMs revealed no alterations in apoptosis (wt 0.75 ± 0.70%, n = 4, bal 1.07 ± 1.28%, n = 6, p = 0.92), error bars indicate s.d., ns, not significant. (H, I) IF against Mef2 (red) and EdU (green) on dissected wt and bal hearts showed decreased numbers of EdU⁺ ventricular CMs. (J) Quantification revealed significantly decreased levels of EdU⁺ ventricular CMs in bal mutant hearts (wt 4.10 ± 2.73, n = 9, bal 0.61 ± 0.92, n = 11, p = 0.001). For significance testing Mann-Whitney-U test was applied, error bars indicate s.d., ***p < 0.001, ns, not significant.

Expression Data

Genes:	cmlc1 myh7
Antibody:	Ab1-mef2
Fish:	AB hdac1^{ulm107/ulm107} (AB)
Anatomical Terms:	cardiac muscle cell cardiac muscle myoblast
Stage Range:	14-19 somites to Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	hdac1^{ulm107/ulm107} (AB)
Observed In:	cardiac muscle cell apoptotic process cardiac muscle cell cell population proliferation
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS Genet.