FIGURE

Figure 5

ID
ZDB-FIG-211029-215
Publication
Dona et al., 2021 - Targeting the Redox Balance Pathway Using Ascorbic Acid in sdhb Zebrafish Mutant Larvae
Other Figures
All Figure Page
Back to All Figure Page
Figure 5

Startle response measurements at basal level and after low- and high-dosage levels of Vitamin C. (A) Optimized short protocol (<5 min in total) to quickly assess startle responses as a toxicity indicator induced by tapping stimuli with random intervals varying between 2 and 35 s. The max distance moved (mm min−1) is plotted against time per seconds. Between the wild-type (green line) and heterozygous mutants (blue line), no differences were observed, while homozygous sdhb mutants (red line) showed a decrease in moved distance. (B) Quantification of the average of the maximum velocity of three startle responses with or without the supplementation of low-dosage levels of Vitamin C (20 mg⋅L−1). Low-dosage levels of Vitamin C decreased the startle response of wild-type and heterozygous sdhb larvae while not affecting homozygous sdhb larvae. Larvae were supplemented with 20 mg⋅L−1 Vitamin C homozygous sdhb (n = 106), heterozygous sibling (n = 244), and wild-type sibling (n = 137) compared to the control group (indicated with 0 mg⋅L−1) consisting of homozygous sdhb larvae (n = 91), heterozygous sibling (n = 227), and wild-type sibling (n = 100) from five different replicates measured at 6 dpf. (C) Quantification of the average of the maximum velocity of three startle responses with or without supplementation of high-dosage levels of Vitamin C (500 and 1000 mg⋅L−1). Both 500 and 1000 mg⋅L−1 concentrations of Vitamin C induced a decreased startle response in heterozygous sdhb larvae, while only the 1000 mg⋅L−1 concentration of Vitamin C induced a decreased startle response in homozygous sdhb larvae and none of the high-dosage levels of Vitamin C significantly altered the startle response of the wild-type larvae. Larvae were supplemented with 500 mg⋅L−1 Vitamin C homozygous sdhb (n = 73), heterozygous sibling (n = 139), and wild-type sibling (n = 55), 1000 mg⋅L−1 Vitamin C homozygous sdhb (n = 20), heterozygous sibling (n = 37), and wild-type sibling (n = 19) compared to the control group (indicated with 0 mg⋅L−1) consisting of homozygous sdhb larvae (n = 32), heterozygous sibling (n = 48), and wild-type sibling (n = 40) from at least two different replicates measured at 6 dpf. One-way ANOVA with Tukey’s post hoc test, * p < 0.05 and *** p < 0.001.

Expression Data

Expression Detail
Antibody Labeling
Phenotype Data
Fish:
Condition:
Observed In:
Stage: Day 6

Phenotype Detail
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Cancers