FIGURE

Figure 4

ID

ZDB-FIG-211029-130

Publication

Robinson et al., 2021 - A Novel Calpain Inhibitor Compound Has Protective Effects on a Zebrafish Model of Spinocerebellar Ataxia Type 3

Other Figures

All Figure Page

Back to All Figure Page

Figure 4

Treatment with BLD-2736 increased the activity of the autophagy protein quality control pathway. (A) Immunoblotting of LC3II revealed changes in the levels of LC3II relative to GAPDH across treatment groups. (B) Quantitative analysis of the ratio of LC3II to GAPDH was found to be significantly increased in larvae treated with 300 nM BLD-2736 when compared to larvae treated with the autophagy inhibitor chloroquine and 300 nM BLD-2736, indicating increased synthesis of LC3II and induction of autophagy. Protein lysates were produced from pooled samples of n = 12–20 zebrafish larvae. Each data point represents an independent experimental replicate. All data are presented as mean ± SEM. * represents p < 0.05, ** represents p < 0.01, **** represents p < 0.0001.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Cells