FIGURE

Fig. 2.

ID

ZDB-FIG-211025-92

Publication

de Bakker et al., 2021 - Prrx1b restricts fibrosis and promotes Nrg1-dependent cardiomyocyte proliferation during zebrafish heart regeneration

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Fig. 2.

Prrx1 is expressed in the epicardium/EPDCs and follows epicardial dynamics post-injury. (A) Schematic illustrating the experimental procedures. (B-F) Immunofluorescence staining on 1, 3, 7, 14 and 30 dpi tcf21:mCherry⁺ wild-type heart sections staining Prrx1 (green) and mCherry (magenta). Areas in the coloured boxes are shown at higher magnification below. Arrowheads indicate tcf21:mCherry⁺/Prrx1⁺ cells. Scale bars: 100 μm (low-magnification images); 10 μm (high-magnification images). BZ epicardium, border zone epicardium; Rm epicardium, remote epicardium. Six hearts analysed per condition. Dashed line indicates the border between myocardium and injury area. (G) Schematic of Prrx1 dynamics upon injury. Prrx1⁺ cells are in green. Dark colour at the apex represents the injury area. (H) Quantification of the distribution of tcf21:mCherry⁺/Prrx1⁺ cells per time point. Size of the dots represents the percentage of tcf21:mCherry⁺/Prrx1⁺ cells and absolute count number is visualized by a colour gradient.

Expression Data

Gene:	mCherry
Antibody:	Ab1-prrx1
Fish:	cz1701Tg/cz1701Tg; pd42Tg/pd42Tg
Condition:	chemical treatment by environment: tamoxifen, cold damage: heart
Anatomical Term:	ventricular epicardium
Stage:	Adult

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	cz1701Tg/cz1701Tg; pd42Tg/pd42Tg
Condition:	chemical treatment by environment: tamoxifen, cold damage: heart
Observed In:	ventricular epicardium wound healing, spreading of cells
Stage:	Adult

Phenotype Detail

Acknowledgments

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