FIGURE

Fig. 2.

ID

ZDB-FIG-211025-102

Publication

Robinson et al., 2021 - Flow cytometry allows rapid detection of protein aggregates in cellular and zebrafish models of spinocerebellar ataxia 3

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Fig. 2.

Flow cytometry can be used to quantify the number of detergent-insoluble EGFP ataxin-3 particles relative to the number of nuclei. (A) Representative flow cytometry scatterplots displaying UV⁺ nuclei and EGFP-fused ataxin-3 particles from cell lysates. (B) Quantification of the number of DAPI⁺ nuclei revealed that similar numbers of nuclei were present in the analysed samples. (C) Cells expressing EGFP-fused mutant ataxin-3 (84 glutamines) developed significantly more insoluble particles compared to EGFP alone or EGFP-fused ataxin-3 with a short polyglutamine stretch (28 glutamines). (D) Detergent-insoluble particle size did not significantly differ across the examined genotypes. Data are mean±s.e.m. n=4-9 experimental replicates. *P<0.05; **P<0.01; ns, not significant (one-way ANOVA with Tukey's post-hoc test). AU, arbitrary units; RFU, relative fluorescence units.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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