FIGURE 3

Growth performance and feed intake of gh-transgenic zebrafish (D. rerio). Body weight (A), length (B), SGR (C), weight gain (D), and feed intake (E) were significantly increased in response to GH excess in transgenic zebrafish compared to non-transgenic full siblings. Weight (mg) and length (mm) were measured every 15 days during the 60-day growth experiment (non-transgenic, n = 38, and gh-transgenic fish, n = 37). Differences in body weight between the two groups were determined by linear regression analysis. ANOVA with multiple comparisons was performed between the two genotypes at each time point for weight gain and body length. Comparisons of SGR and feed intake between two groups were taken by a two-tailed t-test. Data are shown as means ± SE of 37 fish per group. Asterisks indicate significant differences (*p < 0.05; **p < 0.01; ***p < 0.001; and ****p < 0.0001). Zebrafish that overexpress GH exhibited a linear increase in growth (p < 0.05). gh, growth hormone gene; NT, Non-Transgenic; T, Transgenic.