FIGURE

Figure 2

ID

ZDB-FIG-210919-33

Publication

Yu et al., 2021 - Claudin-5 Affects Endothelial Autophagy in Response to Early Hypoxia

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Figure 2

Loss of Claudin-5b in zebrafish embryos inhibits activation of autophagy in cerebrovascular endothelial cells (ECs). Morpholino (MO) silencing of claudin-5b in endothelial eGFP-specific transgenic Tg(kdrl:eGFP) zebrafish embryos. (A) Brain phenotype (red arrowheads) of control MO and claudin-5b MO zebrafish larvae in response to hypoxia. Scale bar, 100μm. (B) Quantitative analysis of the severe injury rates of control MO and claudin-5b MO zebrafish larval brains. Mean±SD, n=3 independent experiments per group. (C) Confocal microscopy images of Claudin-5 (red) and LC3 (purple). Scale bar, 5μm. (D) Quantitative analysis of LC3 levels by Image-Pro Plus. Mean±SD, n=3 independent experiments per group. ^*p<0.05 and ^**p<0.01.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front. Physiol.