FIGURE

Figure 1

ID

ZDB-FIG-210827-1

Publication

Sitaraman et al., 2021 - Gjd2b-mediated gap junctions promote glutamatergic synapse formation and dendritic elaboration in Purkinje neurons

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Figure 1

Knocking down Gjd2b reduces glutamatergic miniature excitatory postsynaptic current (mEPSC) frequency in Purkinje neurons (PNs) by potentially decreasing synaptic number.

(A) Raw traces of mEPSC recordings from PNs in control (black) and Gjd2b-MO-injected (red) larvae. (B) Average mEPSC waveforms from control and Gjd2b-MO-injected larvae. Inset: scaled mEPSC to show faster decay time of mEPSCs in morphants. Neurons were held at −65 mV. (C–F) Cumulative probability distributions and boxplots of mEPSC inter-event intervals (C), peak amplitudes (D), 10–90% rise times (E), and decay tau (F) in control (black) and Gjd2b-MO-injected (red) larvae. N = 7 cells from 7 larvae from 5 clutches for control and 12 cells from 12 larvae from 8 clutches for the Gjd2b-MO group. **p<0.01; ***p<0.001; ****p<0.0001; Mann–Whitney U test. See also Figure 1—figure supplements 1 and 2. Data used for quantitative analyses are available in Figure 1—source data 1.

Expression Data

Gene:	Venus
Antibody:	Ab1-cx35
Fish:	IND IND + MO1-gjd2b rk22Tg (IND)
Knockdown Reagent:	MO1-gjd2b
Anatomical Terms:	cerebellum Purkinje cell cell body membrane Purkinje cell dendrite
Stage:	Day 6

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	IND + MO1-gjd2b
Knockdown Reagent:	MO1-gjd2b
Observed In:	cerebellum Purkinje cell action potential initiation
Stage Range:	Day 6 to Days 7-13

Phenotype Detail

Acknowledgments

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