FIGURE

FIGURE 4

ID

ZDB-FIG-210725-4

Publication

Shanaka et al., 2021 - Growth and Antioxidant-Related Effects of the Reestablished Ascorbic Acid Pathway in Zebrafish (Danio rerio) by Genomic Integration of L-Gulonolactone Oxidase From Cloudy Catshark (Scyliorhinus torazame)

Other Figures

All Figure Page

Back to All Figure Page

FIGURE 4

The sGULO enzyme activity and AA level in Wt and Tg fish. (A) Detection of sGULO activity in Tg and Wt fish. Whole zebrafish were homogenized and used to detect the endogenous enzyme activity. This experiment was performed using an Oxi Select AA detection kit. The production rate of AA in Wt and Tg fish was measured after the addition of gulonolactone. (B) Detection of the endogenous AA level in Wt and Tg fish. Whole zebrafish were homogenized and used to detect endogenous AA levels using an Oxi Select AA detection kit. The error bars represent the standard deviation associated with experimental triplicates. The statistical significance between the Wt and Tg groups was analyzed using a t-test, and the significance is indicated by an asterisk *p < 0.05.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front. Physiol.