Fig. 3.

Alternative splicing of mbnl1 exon 5 was misregulated across tissues in zebrafish mbnl mutants. (A-F) RT-PCR analysis showing percentage spliced in (PSI) of mbnl1 exon 5 in WT and mbnl mutant whole 5 dpf larvae (A) and in adult skeletal muscle (B), heart (C), brain (D), cornea (E) and intestine (F). mbnl1 mutant alleles are denoted as 1^A and 1^B, mbnl2 alleles as 2^A, and mbnl3 alleles as 3^A, 3^B and 3^C. Representative RT-PCR gels are shown above each graph with band sizes in bp on the left. White boxes represent constitutive exons and gray boxes represent alternative exons. Dividing lines indicate samples run on separate gels. MBNL1 exon 5 inclusion is increased in human DM1 patients and other DM models (Gates et al., 2011; Terenzi and Ladd, 2010; Tran et al., 2011). In A-F, each dot represents RNA from one adult fish or a pool of five larval fish. Data are presented as mean±s.e.m. Data were analyzed by ordinary one-way ANOVA with Tukey's multiple comparisons test. Data bars that do not share the same letter above them are significantly different from one another. Raw data and statistical analysis details are in Table S5.