FIGURE

Fig. 7

ID

ZDB-FIG-210703-17

Publication

Mauri et al., 2021 - The adaptor protein Grb2b is an essential modulator for lympho-venous sprout formation in the zebrafish trunk

Other Figures

All Figure Page

Back to All Figure Page

Fig. 7

In the absence of arteries, cells fail to sprout in grb2b mutants. a–c Confocal projections of plcγ-1 MO injected embryos. All vessels are shown in green (fli:GFP). d Sprout quantification at 48hpf of embryos from a tabula rasa in-cross, injected with a plcγ-1 MO. plcγ-1 MO injection inhibits primary sprouting, allowing quantification/visualization of secondary sprouts in absence of arteries. The number of lympho-venous sprouts is significantly decreased in tabula rasa mutants. wt: n = 4; het: n = 32; mut: n = 24. ****Between wt and mut: P value < 0.0001 (Mann–Whitney); ****Between het and mut: P value < 0.0001 (Mann–Whitney); ns not significant. MO morpholino. Scale bars: 50 µm. Data in d are mean ± sd

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	grb2b^{hu6987/hu6987}; y1Tg + MO3-plcg1
Knockdown Reagent:	MO3-plcg1
Observed In:	sprouting angiogenesis
Stage:	Long-pec

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Angiogenesis