Multispecies proteome mapping across cardiac chambers.(a) Workflow for the determination of chamber-specific cardiac proteomes in human, horse, pig, rat, mouse, and zebrafish. Tissue biopsies were collected in triplicates. Biopsies were homogenized followed by protein extraction and digestion, desalted peptides were then fractionated, and the generated peptide fractions were analyzed by LC–MS/MS. Data were analyzed using MaxQuant and Perseus software. (b) Table summarizing the number of proteins measured in each species across cardiac chambers. HPLC, high-pressure liquid chromatography; LA, left atrium; LC–MS/MS, liquid chromatography tandem mass spectrometry; LV, left ventricle; RA, right atrium; RV, right ventricle.
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