FIGURE

Figure 6

ID

ZDB-FIG-210327-11

Publication

Sulliman et al., 2021 - HDL biodistribution and brain receptors in zebrafish, using HDLs as vectors for targeting endothelial cells and neural progenitors

Other Figures

All Figure Page

Back to All Figure Page

Figure 6

HDLs are taken up by the lesioned hemisphere after stab wound injury in zebrafish. ApoA-I immunohistochemistry (green) on injured zebrafish brain following intraperitoneal injection with 80 mg/kg of red fluorescent reconstituted HDLs (n = 8) or PBS (n = 6) as control. The design of the experiment is presented at the top of the figure. (A–L) Note that red fluorescence (HDL) and ApoA-I staining (green) are localized within the brain parenchyma of the injured hemisphere, while HDLs are clearly located in the cerebral vasculature in the contralateral (uninjured) hemisphere. The arrows indicate ApoA-I staining in ventricular cells, probably corresponding to NSCs. In PBS-injected fish (n = 3), ApoA-I immunohistochemistry did not provide any staining, demonstrating the specificity of the labeling. (M–N) ApoA-I immunohistochemistry on GFAP::GFP transgenic fish⁵⁵ injected with HDLs immediately after the lesion. The arrows show GFAP-positive NSCs co-labeled with ApoA-I. Scale bar = 200 μm (A–H) and 20 μm (I, J).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Sci. Rep.