FIGURE

Fig. 5

ID

ZDB-FIG-210324-34

Publication

Olguin-Olguin et al., 2021 - Chemokine-biased robust self-organizing polarization of migrating cells in vivo

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Fig. 5

Chemokine-directed polarity establishment. (A) A scheme presenting the generation of local chemokine source within the live embryo. (B–D) Apolar cells (Left), the emergence of the polarized front (time 00:00) (Middle), and the subsequent polarized localization of the rear markers (Right). Lifeact is labeled with magenta and rear markers (B) Ezrin, (C) Esyt2a, and (D) MTs (Clip170H) are in green. White arrows indicate the direction of migration toward the chemokine source. Time is in minutes and seconds. (Scale bar, 10 µm.) (B′–D′) Time course of polarity index establishment during cell polarization; time is in seconds. For B′ and C′ the signal was measured only at the level of the membrane. In B–D the distribution of the chemokine (Cxcl12a) is illustrated by the red gradient below the panels. (E) Timing of the chemokine induced polarization steps relative to the time of actin localization at the cell front (time 0) (median, 5 and 95 percentiles are presented, n = number of cells, time in seconds).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA