FIGURE

Figure 6

ID

ZDB-FIG-210310-70

Publication

Lu et al., 2021 - Generation of Knockout and Transgenic Zebrafish to Characterize Abcc4 Functions in Detoxification and Efflux of Lead

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Figure 6

Functional characterization of Abcc4 in lead detoxification in transgenic zebrafish. (A) Survival rates of abcc4-transgenic and WT embryos were monitored after treatment with different concentrations of lead (0–600 μM) from 96 to 168 hpf. (B) Survival rates of abcc4-transgenic and WT larvae were detected after exposure to 400 μM lead for 72 h. (C) Contents of lead in abcc4-transgenic and WT larvae after exposure to 10 μM lead with or without 1 mM glutathione (GSH) at the indicated exposure time points. (D) Contents of lead in abcc4-transgenic and WT larvae at the indicated exposure time points after treatment with a medium containing 10 μM lead and simultaneously with 5 μM buthionine sulfoximine (BSO), an inhibitor of GSH biosynthesis. (E) Intracellular GSH contents in abcc4-transgenic and WT larvae exposed to 10 μM lead at the indicated exposure time points. (F) ATPase activities as shown by inorganic phosphate (Pi) levels in abcc4-transgenic and WT larvae after treatment with 10 μM lead at the indicated exposure time points. Values are expressed as means ± SD (n = 3). Significant differences are indicated by * p < 0.05 and ** p < 0.01.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	ihb367Tg/+ (AB) AB
Condition:	chemical treatment by environment: lead(0)
Observed In:	ATP hydrolysis activity whole organism whole organism glutathione whole organism lead(0)
Stage:	Days 7-13

Phenotype Detail

Acknowledgments

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