FIGURE

FIGURE 7

ID

ZDB-FIG-210310-30

Publication

Ranawakage et al., 2021 - Efficient CRISPR-Cas9-Mediated Knock-In of Composite Tags in Zebrafish Using Long ssDNA as a Donor

Other Figures

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FIGURE 7

Expression of the tagged Sox3 protein. (A) Western blot and HiBiT blot analyses of the tagged Sox3 protein. Two-color Western blot analysis of sox3 knock-in embryos derived from the FLAGx3-50_#16 (a) and PAx3-50_#21 founders (b) with antibodies against Sox3, FLAG tag or PA tag, and α-tubulin. HiBiT blotting using an excessive amount of LgBiT protein and substrate to detect the C-terminal HiBiT peptide of the tagged Sox3 proteins (c). (B) Whole-mount immunohistochemical staining for the tagged Sox3 protein. Each primary antibody used to stain zebrafish is indicated below each image. Genotyping was conducted using PCR with genomic DNA prepared from the immunostained embryos after image acquisition.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front Cell Dev Biol