FIGURE 2

Ganglion cell competence factor atoh7 is upregulated in the light-damaged retina. (Aa–aj) Single z-plane confocal images of retinal sections from light-damaged Tg[atoh7:GFP]rw021 zebrafish (0, 48, 60, 72, 84, 96 hLT) immunolabeled for PCNA (Aa–f,As–ad), GFP (Ag–l,As–aj), HuC/D (Am–x,Aae–aj) and counterstained with DAPI (As–x). (Ay–aj) Regions outlined in panels (As–x) at higher magnification. Yellow arrows, atoh7:GFP, PCNA and HuC/D-triple positive cells. Red outlined arrowheads, atoh7:GFP and HuC/D-double positive cell that is PCNA-negative. Scale bars, 20 μm (Aa) and 10 μm (Ay). (B–D) Number of PCNA-positive, atoh7:GFP-positive and PCNA and atoh7:GFP-double positive cells in the INL (B), ONL (C), and GCL (D) over the light treatment timecourse. (E–G) Number of atoh7:GFP-positive cells and atoh7:GFP and PCNA−double positive cells in comparison to atoh7:GFP and HuC/D-double positive and PCNA and HuC/D-double positive cells in the INL (E), ONL (F), and GCL (G) of retinas exposed to constant intense light for 0, 36, 48, 60, 72, 84, and 96 h. Mean ± SE, n ≥ 12, *p_Tukey < 0.05 and ^#p < 0.05 indicate comparisons to 0 hLT for the different measures that were assessed. The symbols are color-coded according to the line that they represent in the corresponding graphs (p_ANOVA, see Table 1). Significance was not determined for PCNA in panels (B–D) and symbols indicating significance for atoh7:GFP and atoh7:GFP and PCNA-double-positive cells are not shown in panels (E–G), as they are indicated in panels (B–D). PCNA, Proliferating Cell Nuclear Antigen.