The A. nidulans clinical isolates MO80069 and SP-2605-48 do not present increased survival in the presence of macrophages and neutrophils. (A) Percentage of phagocytized conidia by murine wild-type and gp91phox knockout macrophages. Macrophages were incubated for 1.5 h with conidia from the respective strains before phagocytized conidia were counted. (B) CFU counts as a measure of conidium viability after passage through wild-type (wt) and gp91phox knockout macrophages. Macrophages were incubated with the respective conidia for 1.5 h before they were lysed, and contents were plated on complete medium. (C) Percentage of viable hyphal germlings after incubation for 16 h with neutrophils from healthy human donors. Strain viability was calculated relative to incubation without PMN cells, which was set at 100% for each sample. Standard deviations represent biological triplicates in a one-way ANOVA test with Tukey’s posttest (*, P < 0.05; **, P < 0.01, for results for the clinical isolates compared to those for FGSC A4; #, P < 0.05, for a comparison of results for the two types of macrophages in the same strain).
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